Under CopyrightMandal, M.K.M.K.Mandal2022-03-0723.09.20122011https://publica.fraunhofer.de/handle/publica/27899610.24406/publica-fhg-278996The first endogenous matrix-metalloproteinase NtMMP1 from tobacco BY-2 cells was characterized in detail. Transgenic BY-2 cell lines expressing antisense-RNA against different endogenous proteases (aspartic, cysteine, matrix-metallo and serine) separately (single-antisense) or simultaneously (multi-antisense) was generated. The inhibition of endogenous protease(s) in those lines was verified and the stability of recombinant protein(s) was tested.enAngewandte Forschungapplied research540doctoral thesis