Paul, D.D.PaulStadler, H.H.Stadler2022-03-082022-03-081999https://publica.fraunhofer.de/handle/publica/304244NOVELTY - A screening process to isolate an X-mer oligonucleotide from a library, corresponding to all the possible X-mers (4x), is characterized in that the library is divided into 256 sublibraries with defined sequences in positions X, X1, X2 and X3, where one of these libraries binds with a target, the winner corresponds to another library divided into 256 sublibraries with defined sequences at positions X4, X5, X6 and X7, where one of these libraries binds with a target and the process is repeated, to determine the whole sequence. USE - The method is used to produce new oligonucleotides and their use in the pharmaceutical industry and for medicines. Oligonucleotides are selected by their binding to a target that is known, such as growth factors, interaction domains of cell cycle proteins and viral proteins. The oligonucleotides may be used as ribozymes.de608615610620patent1997-19757950