Wagner, JulianeJulianeWagnerBayer, LennartLennartBayerLoger, KlaasKlaasLogerAcil, YahyaYahyaAcilKurz, SaschaSaschaKurzSpille, JohannesJohannesSpilleIngwersen, Lena-ChristinLena-ChristinIngwersenAhlhelm, MatthiasMatthiasAhlhelmJonitz-Heincke, AnikaAnikaJonitz-HeinckeSedaghat, SamSamSedaghatWiltfang, JörgJörgWiltfangNaujokat, HendrikHendrikNaujokat2024-12-022024-12-022024-11https://publica.fraunhofer.de/handle/publica/47951610.1016/j.jcms.2024.04.012Purpose: Correction of bony mandibular defects is a challenge in oral and maxillofacial surgery due to aesthetic and functional requirements. This study investigated the potential of a novel hybrid scaffold for bone regeneration and degradation assessment of the ceramic within the omentum majus over 6 months and the extent to which rhBMP-2 as a growth factor, alone or combined with a hydrogel, affects regeneration. Materials and methods: In this animal study, 10 Göttingen minipigs each had one scaffold implanted in the greater omentum. Five animals had scaffolds loaded with a collagen hydrogel and rhBMP-2, and the other five animals (control group) had scaffolds loaded with rhBMP-2 only. Fluorochrome injections and computed tomography (CT) were performed regularly. After 6 months, the animals were euthanized, and samples were collected for microCT and histological evaluations. Results: Fluorescent and light microscopic and a CT morphological density evaluation showed continuous bone growth until week 16 in both groups. Regarding the ratio of bone attachment to the Zr02 support struts, the rhBMP-2 loaded collagen hydrogel group showed with 63% a significantly higher attachment (p > 0.001) than the rhBMP-2 control group (49%). Conclusion: In this study, bone growth was induced in all omentum majus specimens until post-operative week 16. Furthermore, hydrogel and rhBMP-2 together resulted in better bone-scaffold integration than rhBMP-2 alone. Further studies should investigate whether implantation of the scaffolds in the jaw after an appropriate period of bone regeneration leads to a stable situation and the desired results.enBone tissue engineeringEndocultivationFacial bone regenerationScaffolds600 Technik, Medizin, angewandte Wissenschaftenjournal article