Dienerowitz, M.M.DienerowitzHeitkamp, T.T.HeitkampGottschall, T.T.GottschallLimpert, J.J.LimpertBörsch, M.M.Börsch2022-03-132022-03-132017https://publica.fraunhofer.de/handle/publica/39858510.1117/12.2250550Trapping nanoscopic objects to observe their dynamic behaviour for extended periods of time is an ongoing quest. Particularly, sub-100nm transparent objects are hard to catch and most techniques rely on immobilisation or transient diffusion through a confocal laser focus. We present an Anti-Brownian ELectrokinetic trap1-7 (pioneered by A. E. Cohen and W. E. Moerner) to hold nanoparticles and individual FoF1-ATP synthase proteins in solution. We are interested in the conformational dynamics of this membrane-bound rotary motor protein that we monitor using single-molecule FRET. The ABELtrap is an active feedback system cancelling the nano-object's Brownian motion by applying an electric field. We show how the induced electrokinetic forces confine the motion of nanoparticles and proteoliposomes to the centre of the trap.en620conference paper