CC BY 4.0Schmohl, AndreasAndreasSchmohlNagele-Renzl, Anna MariaAnna MariaNagele-RenzlBuschhaus, MichaelMichaelBuschhaus2025-08-292025-08-292025-07-09https://publica.fraunhofer.de/handle/publica/494713https://doi.org/10.24406/publica-521710.3390/environments1207023310.24406/publica-5217Ultraviolet (UV) radiation can be used to inactivate microorganisms, with upper-room UV germicidal irradiation (UR-UVGI) representing a promising approach. This study investigated the inactivation of the airborne surrogate virus Phi6 by a UR-UVGI system based on light-emitting diodes (LEDs) in a realistic test setup. Two test scenarios were used, one with continuous Phi6 release, simulating a source located in the room and leading to a dynamic equilibrium, and the second simulating a situation in which the source has left the room and an exponential decay is evaluated. The "Incremental Evaluation Model" was adapted and used to evaluate the dynamic equilibrium measurement. At a position in the breathing direction 5 m away from the Phi6 source, the loss coefficient (air exchange rate) was 25 h-1 in the first scenario and 30 h-1 in the second. These results show that UR-UVGI systems can effectively inactivate microorganisms. However, at 1 m distance from the Phi6 source perpendicular to the breathing direction, only minimal inactivation was observed due to short-circuit airflow. At this position, the loss coefficient was <2 h-1 in the first scenario and 17 h-1 in the second scenario, indicating that short-circuit airflows can only be detected by dynamic equilibrium measurements.engermicidal ultravioletGUVupper airupper spacevirus inactivationbacteriophage Phi6air purifierplaque assayindoor air qualitythermal convectionjournal article