Juzenas, S.S.JuzenasVenkatesh, G.G.VenkateshHübenthal, M.M.HübenthalHoeppner, M.P.M.P.HoeppnerDu, Z.G.Z.G.DuPaulsen, M.M.PaulsenRosenstiel, P.P.RosenstielSenger, P.P.SengerHofmann-Apitius, M.M.Hofmann-ApitiusKeller, A.A.KellerKupcinskas, L.L.KupcinskasFranke, A.A.FrankeHemmrich-Stanisak, G.G.Hemmrich-Stanisak2022-03-052022-03-052017https://publica.fraunhofer.de/handle/publica/25126510.1093/nar/gkx706With this study, we provide a comprehensive reference dataset of detailed miRNA expression profiles from seven types of human peripheral blood cells (NK cells, B lymphocytes, cytotoxic T lymphocytes, T helper cells,monocytes, neutrophils and erythrocytes), serum, exosomes and whole blood. The peripheral blood cells from buffy coats were typed and sorted using FACS/MACS. The overall dataset was generated from 450 small RNA libraries using high-throughput sequencing. By employing a comprehensive bioinformatics and statistical analysis, we show that 3' trimming modifications as well as composition of 3' added non-templated nucleotides are distributed in a lineage-specific manner-the closer the hematopoietic progenitors are, the higher their similarities in sequence variation of the 3' end. Furthermore, we define the blood cell-specific miRNA and isomiR expression patterns and identify novel cell type specific miRNA candidates. The study provides the most comprehensive cont ribution to date towards a complete miRNA catalogue of human peripheral blood, which can be used as a reference for future studies. The dataset has been deposited in GEO and also can be explored interactively following this link: http://134.245.63.235/ikmb-tools/bloodmiRs.en572journal article