Wolf, MariusMariusWolfHanstein, StefanStefanHanstein2024-08-262024-08-262024https://publica.fraunhofer.de/handle/publica/47407010.1002/cite.2023001922-s2.0-85185938086Extraction of hemicelluloses from biomass with pressurized hot-water employs the mechanism of autohydrolysis for molecular weight reduction. In a pilot-scale extraction of depectinized apple pomace, molecular weight was 30 kDa with a broad mass distribution including monomers and their degradation products. Ultrafiltration with sequential use of ceramic membranes with decreasing cut-off yielded a suitable fraction with molecular weight of 9 kDa representing 10 % of the extracted hemicelluloses. Further autohydrolytic treatment of the extracted hemicellulose decreases molecular weight while increasing the mass of degradation products. For a series of hydrolysates sequential filtration through ceramic membranes was further tested for fractionation and purification. The efficiency of this fractionation was analyzed by size exclusion chromatography and a total mass balance of the ultrafiltration process is given as an evaluation for suitability of purifying and fraction glycans.enopen accessBiomass valorizationCeramic ultrafiltration membranesHemicelluloseSEC-MALSSize distribution analysisjournal article