Pretzsch, F.F.PretzschKoropp, U.U.KoroppMertsching, H.H.MertschingKaufmann, M.M.KaufmannHansmann, JanJanHansmann2022-03-082022-03-082009https://publica.fraunhofer.de/handle/publica/308867(A1) Die Erfindung betrifft Verfahren zur Abtrennung von in Suspensionen suspendierten Partikeln, wobei bevorzugt gleichzeitig eine vorbestimmte Partikelkonzentration in einer Suspension eingestellt und eine Partikelsuspension mit eingestellter Konzentration erhalten werden kann. Die Erfindung betrifft Mittel zur Durchfuehrung dieses Verfahrens. Ein besonderes Anwendungsgebiet sind Suspensionen biologischer Zellen und deren GMP-konforme vollautomatische Verarbeitung.DE 102009022353 A1 UPAB: 20101130 NOVELTY - The method for isolation of particles (110) suspended in a first suspension from the suspension, where the first suspension comprises further suspended components, comprises separating the particles from the first suspension through filtration in two filters (420, 430), where the first filter is suited for the retention of the further component and for the filtration of the particles to be isolated from the first suspension and the further component is retained at the first filter and adhering the retained particles at the second filter. DETAILED DESCRIPTION - The method for isolation of particles (110) suspended in a first suspension from the suspension, where the first suspension comprises further suspended components, comprises separating the particles from the first suspension through filtration in two filters (420, 430), where the first filter is suited for the retention of the further component and for the filtration of the particles to be isolated from the first suspension and the further component is retained at the first filter, adhering the retained particles at the second filter, removing the first filter with the further component of the suspension, resuspending the particles at the second filter in a defined first volume of a second suspending fluid, where the second suspension of the particle is obtained, determining the total number of the particle to be isolated in the second suspension or number of the particles in part volume, separating the particles to be isolated from the second suspension through renewed retaining of the particle in the second filter, and resuspending the particles retained in the second filter in a second volume of a third suspending fluid, where a third suspension of the particle is obtained. The second volume of the third suspending fluid is selected in dependent upon the number of particles determined in the determination step and the pre-determined concentration of the particle is adjusted in the third suspension. The suspending fluid of the first suspension is filtered through applying the pressure drops by two filters. The particles to be isolated are held by applying a pressure drop over the second filter on the filter. The resuspending of the particle is carried out through backwashing the second filters with the second suspending fluid in a surrounded volume for the reception of the second suspension. The surrounded volume is a pipette tip. The determination of the total number of the particles is carried out by transporting a part volume of the second suspension into a counter means and counting the number of particles in the part volume of the second suspension. The second suspending fluid of the second suspension is filtered through the application of the pressure drop by the second filter. The pore size of the second filter is 10 mu m and the pore size of the first filter is 20 mu m. An INDEPENDENT CLAIM is included for a method for the production of particle suspension with pre-determined particle concentration. USE - Method useful for isolation of particles suspended in a first suspension from the suspension. ADVANTAGE - The method ensures simple and efficient isolation of particles from the suspension without the risk of contamination.de658670660610620patent102009022353