Ohlander, A.A.OhlanderBauer, S.S.BauerRamachandraiah, H.H.RamachandraiahRussom, A.A.RussomBock, K.K.Bock2022-03-122022-03-122014https://publica.fraunhofer.de/handle/publica/385634We report on genotyping of single nucleotide polymorphisms (SNP) by melting curve analysis (MCA) on DNA microarrays in a plastic microfluidic system with integrated heaters using lamination foils. Thin-film micro-heaters were processed on polyethylene napthalate (PEN) foil in only one metallization step. DNA microarrays were prepared directly on the PEN surface without the need of surface pre-treatment via UV-mediated immobilization of the spotted DNA microarrays. MCA in microfluidic channels is demonstrated at a ramping rate of 60°C/min, enabling ultra-rapid (50 seconds) SNP analysis.en621conference paper