Böhm, J.J.BöhmKrehle, K.K.KrehleOtto, B.B.Otto2022-03-032022-03-031991https://publica.fraunhofer.de/handle/publica/180448The cDNA coding for human interferon Gamma contains approximately 600 basepairs not translated sequence with 4 adenine/thymidine (AT)-rich clusters at its 3'end. The influence of this sequence on the expression of recombinant human Interferon Gamma in Escherichia coli was investigated. Removal of the non-translated sequence and especially of the AT-rich clusters yielded an increase in expression of interferon Gamma from 10 to 40% of total protein. These results may also be important for the production of other recombinant human proteins with similar 3'sequences in their cDNA.enEscherichia coligenehuman interferon-gammainterferon-gammapharmaceuticproteinrecombinant interferon-gamma615610620journal article