Kreider, A.A.KreiderSell, S.S.SellKowalik, T.T.KowalikHartwig, A.A.HartwigGrunwald, I.I.Grunwald2022-03-042022-03-042014https://publica.fraunhofer.de/handle/publica/23628510.1016/j.colsurfb.2013.07.0222-s2.0-84893821834A new coupling strategy for biomacromolecules with (3-mercaptopropyl)trimethoxysilane (3MPTMS) and 11-(triethoxysilyl)undecanal (TESU) on gold surfaces is. This immobilization protocol was utilized for the enzyme horseradish peroxidase (HRP). To study the reactions and resulting structures, PM-IRRAS measurements were performed. PM-IRRAS shows there is structure preservation of the HRP when the new coupling strategy is used in contrast to non-specific adsorption on gold. The biological activity of adsorbed and immobilized HRP was measured by the enzyme catalyzed oxidation of 3,5,3',5'-tetramethylbenzidine. Covalent immobilization of HRP on TESU film compared to physisorption of HRP shows higher enzyme activity on gold surfaces, confirming the structural preservation detected by PM-IRRAS.enjournal article