Zupke, OliverOliverZupkeDistler, EvaEvaDistlerJürchott, AnnaAnnaJürchottPaiphansiri, UmapornUmapornPaiphansiriDass, MartinMartinDassThomas, SimoneSimoneThomasHartwig, Udo F.Udo F.HartwigTheobald, MatthiasMatthiasTheobaldLandfester, KatharinaKatharinaLandfesterMailänder, VolkerVolkerMailänderHerr, WolfgangWolfgangHerr2022-03-052022-03-052015https://publica.fraunhofer.de/handle/publica/24007310.2217/nnm.14.1602-s2.0-84929104620Aim: T lymphocytes are used as cellular therapeutics in many disease entities including cancer. We investigated the uptake and retention of nanoparticles (NPs) by these nonphagocytic cells. Materials & methods: Uptake, release and toxicity of various polymeric NP preparations were analyzed by flow cytometry, confocal laser scanning microscopy and transmission electron microscopy. T-cell effector functions were measured using IFN-g-ELISPOT and 51Chromium-release assays. Results: Amino-functionalized NPs were efficiently ingested by antigen-specific T cells without adversely influencing effector functions. NPs were stored in membrane-surrounded vesicles, with major proportions released extracellularly during 24 h. Conclusion: Amino-functionalized polymeric NPs are efficiently taken up by human T cells and could be used to design nanocarriers for direct access and manipulation of antigen-specific T cells in vivo.encell imagingcellular therapydrug deliveryleukemiananoparticlesNP releaseT lymphocytestumorjournal article