Fertey, JasminJasminFerteyBayer, LeaLeaBayerKähl, SophieSophieKählHaji, Rukiya M.Rukiya M.HajiBurger-Kentischer, AnkeAnkeBurger-KentischerThoma, MartinMartinThomaStandfest, BastianBastianStandfestSchönfelder, JessiJessiSchönfelderCasado, Javier PortilloJavier PortilloCasadoRögner, Frank-HolmFrank-HolmRögnerBaums, Christoph GeorgChristoph GeorgBaumsGrunwald, ThomasThomasGrunwaldUlbert, SebastianSebastianUlbert2022-06-202022-06-202020https://publica.fraunhofer.de/handle/publica/26183610.3390/vaccines8010113Bacterial pathogens cause severe infections worldwide in livestock and in humans, and antibiotic resistance further increases the importance of prophylactic vaccines. Inactivated bacterial vaccines (bacterins) are usually produced via incubation of the pathogen with chemicals such as formaldehyde, which is time consuming and may cause loss of immunogenicity due to the modification of structural components. We evaluated low-energy electron irradiation (LEEI) as an alternative method to generate a bacterin. Rodentibacter pneumotropicus, an invasive Gram-negative murine pathogen, was inactivated with LEEI and formaldehyde. LEEI resulted in high antigen conservation, and LPS activity was significantly better maintained when compared with formaldehyde treatment. Immunization of mice with LEEI-inactivated R. pneumotropicus elicited a strong immune response with no detectable bacterial burden upon sublethal challenge. The results of this study suggest the inactivation of bacteria with LEEI as an alternative, fast and efficient method to generate bacterial vaccines with increased efficacy.enBacterinElectron beamInactivation of bacteriaLow-energy electron irradiationRodentibacterBakterienElektronenstrahlStrahlentherapieDDC::600 Technik, Medizin, angewandte Wissenschaften::620 IngenieurwissenschaftenDDC::600 Technik, Medizin, angewandte Wissenschaften::660 Chemische VerfahrenstechnikDDC::600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und GesundheitDDC::600 Technik, Medizin, angewandte Wissenschaften::660 Chemische Verfahrenstechnik::667 Reinigungs-, Färbe-, BeschichtungstechnikenDDC::600 Technik, Medizin, angewandte Wissenschaften::670 Industrielle Fertigung620667670journal article