Frasca, S.S.FrascaGraberg, T. vonT. vonGrabergFeng, J.-J.J.-J.FengThomas, A.A.ThomasSmarsly, B.M.B.M.SmarslyWeidinger, I.M.I.M.WeidingerScheller, F.W.F.W.SchellerHildebrandt, P.P.HildebrandtWollenberger, U.U.Wollenberger2022-03-042022-03-042010https://publica.fraunhofer.de/handle/publica/22238710.1002/cctc.2010000472-s2.0-79251529729Stable immobilization and reversible electrochemistry of cytochrome c in a tranparent indium tin oxide film with a well-defined mesoporosity (mpITO) is demonstrated. the transparency and good conductivity, in combination with the large surface area of mpITO, allow the incorporation of a high amount of elelctroactive biomolecules and their electrochemical and spectroscopic investigation. UV/Vis and resonance Raman spectroscopy, in combination with direct protein voltammetry are employed for the characterization of cytochrome c immobilized in the mpITO and reveal no perturbant of the structural of the integrity of the redox protein. The potential of this modified material as a biosensor detection of superoxide anions is also demonstrated.en610540journal article