CC BY 4.0Veres, Tibor Z.Tibor Z.VeresKopcsányi, TámasTámasKopcsányiTirri, MarkoMarkoTirriBraun, ArminArminBraunMiyasaka, MasayukiMasayukiMiyasakaGermain, Ronald N.Ronald N.GermainJalkanen, SirpaSirpaJalkanenSalmi, MarkoMarkoSalmi2022-03-0514.6.20172017https://publica.fraunhofer.de/handle/publica/24862810.1038/s41598-017-00769-610.24406/publica-r-248628The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human small-diameter bronchi. By providing stabilized access to the tracheal mucosa without intubation, our setup uniquely allows dynamic in vivo imaging of mucociliary clearance and steady-state immune cell behavior within the complex airway mucosal tissue.enexperimental models of diseasemultiphoton microscopytime-lapse imagingjournal article