Succinic acid production in fed-batch fermentation of Anaerobiospirillum succiniciproducens using glycerol as carbon source

Considering glycerol as an inexpensive alternative carbon source, the optimal glycerol concentration for succinic acid production with Anaerobiospirillum succiniciproducens was identified in shake-flask trials. The addition of a defined amount of glucose improved the growth and succinic acid productivity significantly. In fed-batch processes with glycerol as sole substrate, a maximum succinic acid concentration and product substrate yield were obtained. The addition of glucose led to a 2.5-fold increased succinic acid concentration whereas the product substrate yield remained nearly constant.