Automated isolation of semi-adherent macrophage-like cells from a fibroblast-contaminated culture using the cell separation robot CellCelector
Abstract
Macrophages are basic effector cells of the immune system and involved in pathogenesis of autoimmune diseases. Homogeneity in cell type is desired for almost every application in research or diagnostics. Contaminants can diminish the reliability, reproducibility or validity of obtained results. Since fibroblasts are common contaminants of primary macrophage cell cultures a method for separation of the cells of interest is required. Using the CellCelector to automatically detect and harvest semi-adherent cells provides a simple alternative for enrichment of macrophages from a fibroblast-contaminated culture without the use of additional substances such as antibodies or proteolytic enzymes.
