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PublicationTeilvorhaben: Automatisierte Biosensoren, selbstlernende Monitoring-Tools und Konzepte für sichere Sensornetzwerke zur Erhöhung der Resilienz von Trinkwasserinfrastrukturen
PublicationControl of drinking water by linking biosensors with physicochemical methodsAn alarm system is developed for the direct detection of toxic substances in drinking water. The vitality of living organisms or cells is monitored continuously by electrochemical and infrared spectroscopic methods. Mammalian cells or bacteria are acting as highly sensitive biosensors and are monitored simultaneously by infrared and electro-chemical techniques. It enables the nonspecific detection of toxins with short response times for implementation in water supplies.
PublicationEnzyme-functionalized biomimetic apatites: Concept and perspectives in view of innovative medical approachesBiomimetic nanocrystalline calcium-deficient apatite compounds are particularly attractive for the setup of bioactive bone-repair scaffolds due to their high similarity to bone mineral in terms of chemical composition, structural and substructural features. As such, along with the increasingly appealing development of moderate temperature engineered routes for sample processing, they have widened the armamentarium of orthopedic and maxillofacial surgeons in the field of bone tissue engineering. This was made possible by exploiting the exceptional surface reactivity of biomimetic apatite nanocrystals, capable of easily exchanging ions or adsorbing (bio)molecules, thus leading to highly-versatile drug delivery systems. In this contribution we focus on the preparation of hybrid materials combining biomimetic nanocrystalline apatites and enzymes (lysozyme and subtilisin). This paper reports physico-chemical data as well as cytotoxicity evaluations towards Cal-72 osteoblast-like cells and finally antimicrobial assessments towards selected strains of interest in bone surgery. Biomimetic apatite/enzyme hybrids could be prepared in varying buffers. They were found to be non-cytotoxic toward osteoblastic cells and the enzymes retained their biological activity (e.g. bond cleavage or antibacterial properties) despite the immobilization and drying processes. Release properties were also examined. Beyond these illustrative examples, the concept of biomimetic apatites functionalized with enzymes is thus shown to be useable in practice, e.g. for antimicrobial purposes, thus widening possible therapeutic perspectives.
PublicationBiofilmvermeidung durch natürliche Wirkstoffe - gezielte und langfristige Freisetzung durch ein PEG-basiertes Depotsystem