Yonsei Fraunhofer IZFP Medical Device Lab YFMED
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PublicationAnti-skeletal muscle atrophy effect of Oenothera odorata root extract via reactive oxygen species-dependent signaling pathways in cellular and mouse model( 2016)
;Lee, Y.-H. ;Kim, W.-J. ;Lee, M.-H. ;Kim, S.-Y. ;Seo, D.-H. ;Kim, H.-S. ;Gelinsky, M.Kim, T.-J.Skeletal muscle atrophy can be defined as a decrease of muscle volume caused by injury or lack of use. This condition is associated with reactive oxygen species (ROS), resulting in various muscular disorders. We acquired 2D and 3D images using micro-computed tomography in gastrocnemius and soleus muscles of sciatic-denervated mice. We confirmed that sciatic denervation-small animal model reduced muscle volume. However, the intraperitoneal injection of Oenothera odorata root extract (EVP) delayed muscle atrophy compared to a control group. We also investigated the mechanism of muscle atrophy's relationship with ROS. EVP suppressed expression of SOD1, and increased expression of HSP70, in both H2O2-treated C2C12 myoblasts and sciatic-denervated mice. Moreover, EVP regulated apoptotic signals, including caspase- 3, Bax, Bcl-2, and ceramide. These results indicate that EVP has a positive effect on reducing the effect of ROS on muscle atrophy.
PublicationCoptis japonica Makino extract suppresses Angiogenesis through regulation of cell cycle-related proteins( 2016)
;Kim, S.H. ;Kim, E.-C. ;Kim, W.-J. ;Lee, M.-H. ;Kim, S.-Y.Kim, T.-J.Angiogenesis, neovascularization from pre-existing vessels, is a key step in tumor growth and metastasis, and anti-angiogenic agents that can interfere with these essential steps of cancer development are a promising strategy for human cancer treatment. In this study, we characterized the anti-angiogenic effects of Coptis japonica Makino extract (CJME) and its mechanism of action. CJME significantly inhibited the proliferation, migration, and invasion of vascular endothelial growth factor (VEGF)-stimulated HUVECs. Furthermore, CJME suppressed VEGF-induced tube formation in vitro and VEGF-induced microvessel sprouting ex vivo. According to our study, CJME blocked VEGF-induced cell cycle transition in G1. CJME decreased expression of cell cycle-regulated proteins, including Cyclin D, Cyclin E, Cdk2, and Cdk4 in response to VEGF.
PublicationEffect of nodakenin on atopic dermatitis-like skin lesions( 2014)
;Park, S.-J. ;Cha, H.-S. ;Lee, Y.-H. ;Kim, W.-J. ;Kim, D.-H. ;Kim, E.-C. ;Lee, K.-H.Kim, T.-J.Nodakenin, derived from the roots of Angelica gigas Nakai, is an important natural resource and medicinal material with anti-allergic and anti-inflammatory activities. We have previously shown that nodakenin inhibits IgE/Ag-induced degranulation in mast cells. In this study, we investigated the inhibitory effect of nodakenin on 2,4-dinitrochloro-benzene (DNCB)-induced atopic dermatitis (AD)-like skin lesions in ICR mice. Scratching behavior, skin severity score, blood IgE level, and skin thickness were improved in DNCB-induced AD-like ICR mice. Our results showed that nodakenin suppressed the increase of AD-like skin lesions in ICR mice. These results suggest that nodakenin may be a potential therapeutic resource for AD as well as an adjunctive agent to control itching associated with AD.
PublicationInhibited apoptosis of C2C12 myoblasts by a eupatorium chinense var. simplicifolium root extract( 2013)
;Lee, J.-H. ;Jung, M.-H. ;Lee, Y.-H. ;Shin, Y. ;Kim, H.-S. ;Schreiber, J.Kim, T.-J.We investigated the effects of a Eupatorium chinense var. simplicifolium (EUC) root extract on muscle disorders and explored the underlying mechanism for oxidative stress-induced C2C12 myoblast damage. An EUC pre-treatment reduced the decreased cell viability after an H2O 2 treatment. The heat shock protein (HSP) 70 level increased, and the phosphorylation of Jun amino-terminal kinases (JNKs) decreased in the EUCpre- treated C2C12 myoblasts. The results of the present study demonstrate the potential benefit of a herbal medicine in treating oxidative stress-related muscle disorders.