Options
1989
Conference Paper
Titel
Charged membrane enzyme reactors for continuous regeneration of native coenzymes. II. Synthesis of hydrophobic compounds with NADP(H) dependent dehydrogenases
Alternative
Enzymreaktoren mit geladenen Membranen fĂ¼r die kontinuierliche Regeneration nativer Coenzyme. II. Synthese hydrophober Verbindungen durch NADP(H)-abhängige Dehydrogenasen
Abstract
Alcohol dehydrogenase, 12-alpha-hydroxysteroid dehydrogenase and glutamate dehydrogenase were succesfully used over long time periods in continuous experiments for stereospecific redox reactions. With alcohol dehydrogenase from Thermoanaerobium brockii the enantiomeric alcohol S-sulcatol was produced which serves as a pheromone. The cofactor NADP(H) was regenerated succesfully with the coupled substrate approach. Important parameters for the economic feasability of the processes are evaluated. With 12-alpha-hydroxysteroid dehydrogenase cholic acid was converted to 12-keto-chenodeoxycholic acid which is an intermediate for a drug for dissolution of gallstones.