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Verfahren zur kombinierten Analyse von Zellinhalten, insbesondere den in biologischen Zellen enthaltenen Nukleinsaeuren sowie Vorrichtung zur Durchfuehrung des Verfahrens

Analyzing cell contents, especially nucleic acids, useful for medical diagnostics.
 
: Kiesewetter, S.; Vohrer, U.; Schuele, A.; Gueth, A.; Michniewski, M.; Dobler, H.; Lindner, H.

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Frontpage ()

DE 1998-19801730 A: 19980119
DE 1998-19801730 A: 19980119
DE 19801730 A1: 19990722
C12Q0001
Deutsch
Patent, Elektronische Publikation
Fraunhofer IPA ()

Abstract
NOVELTY - A process for the analysis of cell contents, especially nucleic acids, uses a substrate specific for the cell component being analyzed bound to macroscopic particles, and ultrasound energy, to produce an analyte with is then subjected to PCR analysis. DETAILED DESCRIPTION - The new process comprises: (1) adding macroscopic particles comprising a cell content-specific substrate on their surface to a buffered cell solution; (2) lyzing the cells and isolating of the cell contents using ultrasound energy; (3) separation and washing of the macroscopic particle-bound cell contents from the buffer solution within the reaction vessel; and (4) drying of the complexes; and (5) amplification and detection of the cell contents using polymerase chain reaction (PCR). An INDEPENDENT CLAIM is also included for a device for carrying out the method described above. USE - The process is used in medical diagnostics. DESCRIPTION OF DRAWING(S) - reaction vessel 1 cell sample 2 buffer solution 3 ma croscopic particles 4 ultrasound energy 5 amplification reagents 6 detection reagents 7 light source 8 vessel resonator 9 cooling medium 10 ultrasound source 11 separation device 12 septum 13 sample volume 14 cells 15 DNA binding substance 16 ultrasound resonator 17 nucleic acids 18 cell fragments 19 release valve 20 .

: http://publica.fraunhofer.de/dokumente/PX-39391.html