Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

Self organization of gastric mucosal cells in culture.

Selbstorganisation von Zellen der Magenschleimhaut in der Zellkultur
: Schwenk, M.; Giebel, J.; Thiedemann, K.-U.

Mechanisms of peptic ulcer healing
Dordrecht: Kluwer Academic Publishers Group, 1991
ISBN: 0-7923-8955-7
Falk-Symposium <59, 1990, Freiburg/Brsg.>
Fraunhofer ITA ( ITEM) ()
cell culture; electron microscopy; Elektronenmikroskopie; gastric mucosa; Magenschleimhaut; ultrastructure; Ultrastruktur; Zellkultur

A cell culture system that allows the study of the self-organization of different epithelial cell types of the guinea pig gastric mucosa into ordered patterns was established. Each of the three major gastric epithelial cell populations shows an individual growth pattern on the culture dishes. In monolayers resulting from unseparated cell suspensions containing a mixture of cells of different types, the different cell species can be distinguished by ultrastructural criteria that are very similar to that of cells of the native gastric mucosa. Each of the cell types participates in the self-organization process that takes place during formation of a monolayer. The interactions between neighbouring cells resemble those present in the intact epithelium in vivo. Cells at the edges of an arteficially wounded monolayer have the tendency to reepithelialize the denuded area. The attachment and self-organization of cells was dependent on the presence of fetal calf serum in the culture medium. Co ating of the culture substrate with components of the extracellular matrix was not necessary.