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Quantitative measurements in continuos-flow HPLC/NMR

: Godejohann, M.; Preiß, A.; Mügge, C.


Analytical chemistry 70 (1998), Nr.3, S.590-595
ISSN: 0003-2700
ISSN: 1520-6882
Fraunhofer ITA ( ITEM) ()
analytical chemistry; Aromatic compounds; liquid chromatography; molecular spectroscopy; Nitro compounds

Two methods for the quantitative determination of compounds in continuous-flow HPLC/NMR are described. The first method uses an internal standard (caffeine) of known concentration directly mixed into the mobile phase, while with the second method, a known amount of internal standard is injected onto the column during the chromatographic run. The latter method was validated using several nitroaromatic compounds and explosives. Deviations between the injected and calculated amounts of analytes are usually below 10 per cent while the relative standard deviation ranges from 2 per cent in the upper microgram range to 40 per cent at the limit of detection.