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NAD-P-H-dependent reduction of aldehyde sugars and aromatic aldehydes by unspecific aldose reductases from yeasts

NAD-P-H-abhängige Reduktion von Aldehydzuckern und aromatischen Aldehyden durch unspezifische Aldosereduktase von Hefen
: Kulbe, K.-D.; Scholze, H.

Deutsche Gesellschaft für Chemisches Apparatewesen, Chemische Technik und Biotechnologie e.V. -DECHEMA-, Frankfurt/Main:
Meeting of Biotechnologists '90
Weinheim: VCH Verlagsgesellschaft, 1990 (Dechema Biotechnology Conferences 4)
ISBN: 3-527-27997-0
S.1015-1018 : Abb.,Lit.
Deutsche Gesellschaft für Chemisches Apparatewesen (Jahrestagung) <1990, Frankfurt/Main>
Fraunhofer IGB ()
Aldosereduktase; Stabilität; Synthese von Polyhydroxyalkohol

A great number of yeast strains have been investigated with reference to their ability for the production of aldose reductase (ALR). All yeasts examined so far required arabinose, ribose of xylose as carbon source for the induction of the enzyme. One of the yeast strains which shows promising aldose reductase activities was selected and ALR was purified 25-fold to a specific activity of about 32 U/mg (NADPH) and 19 U/mg (NADH). Aldose reductase stability is essentially influenced by pH, temperature and by stabilizing ingredients of the storage-buffer. Experiments with the purified enzyme show encouraging results in the synthesis of polyhydroxy alcohols like arabitol, galactiol, mannitol, maltitol, ribitol, sorbitol and xylitol. Expensive coenzyme may be regenerated in a charged ultrafiltration membrane reactor by coupling of aldose reductase with different dehydrogenases.