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Morphogenesis and tropism of organoid vascular pattern in tissue and on interfaces in vitro.

Morphogenese und Tropismus von organoiden vaskulären Mustern in Gewebe und auf Grenzflächen in vitro
: Wissler, J.H.; Chmiel, H.

4th European Symposium on Biomaterials
European Symposium on Biomaterials <4, 1991, Siena>
Fraunhofer IGB ()
angiotropin; biomaterial; Morphogenese; morphogenesis; vascularisation; Vaskularisierung

Vascular morphogenesis, the formation of organoid capillary patterns may result from the interaction of monocytes (macrophages) and endothelial cells. Monocytic morphogen activities for endothelial cells were purified (>100'000 fold) to homogeneity and characterized in structure and function from supernatants of serum-free cultures of isolated, lectin-activated porcine peripheral monocytes and from ischemic/infarcted (inflamed) heart muscle sites prepared by transient coronary occlusion by transfemoral catheterization. An isolated monokine (angiotropin) acts as morphogen in vivo and in vitro in the femto mol range: In tissues of different species, bioactive vascular patterns are induced by sprouting and capillarization phases with vascular leaky tips in transition; a relevance of the reactions in pathophysiological phenomena is suggested and may be exemplified by transient neo-hypervascularization of skeletal muscle (rabbit) with reversible, long-lasting increase in hemodynamics as vis ualized by high 99 Tc-angioscintigrams; as corollary, on defined surfaces, in cultures of cloned endothelial cells, it induces selectively cellular differentiation, migration and spatial organization basic to in-vitro-angiogenesis (1-4). As possible differentiation makers in phenotype changes of endothelial cells, the enzymes D-xylose: NADP 1-oxidoreductase (E.C., polyol: NADP oxidoreductases (aldose reductases, E.C., aldehyde: NAD oxidoreductases (E.C., aldose 1-epimerase (E.C. and carbonate hydro-lyase (carbonic anhydrase, E.C. were investigated (5). The morphogen structure as bioactive copper-ribonucleo-polypeptide complex (Cu-RNP) was determined by its chemical and enzymatic disintegration and modification by HPLC and micro-detection methods (1). Proteolysis of polypeptide and removal of complexing Cu-ion components resulted a RNA (75 bases) (6,7). The RNA was isolated and subjected to chemical sequence analysis. It was found peculia r