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Long-term test with yeast

: Fahrig, R.

Mutation research 203 (1988), Nr.3, S.229
ISSN: 0027-5107
Fraunhofer ITA ( ITEM) ()
carcinogen; carcinogenicity; long-term test; mutagen; mutagenicity; mutagenicity testing; mutation; yeast; yeast cell

A long-term test with yeast strain MP1, suitable for simultaneous detection of mutation, nonreciprocal recombination due to gene conversion, and reciprocal recombination has been developed. Principle of the method is the permanent and exactly calibrated supply of fresh media and removal of exhausted media allowing yeast cells to grow permanently for months. First experiments has shown that it is possible to detect the effect of carcinogens in concentrations being at least 1 order of magnitude lower than in short-term tests. Furthermore it was possible to get positive results with caprolactam, quercetin and chrysarobin, substances which are negative in short-term tests with yeast.