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In vitro biosynthesis and membrane translocation of the serine rich protein of Plasmodium falciparum

: Arnold, H.-H.; Hundt, E.; Knapp, B.; Lingelbach, K.; Ragge, K.; Tümmler, M.

Molecular and Biochemical Parasitology 42 (1990), Nr.1, S.93-100
ISSN: 0166-6851
Fraunhofer ITA ( ITEM) ()
intracellular transport; parasite; parasitology; plasmodium falciparum; secretion; serine-rich protein

The serine-rich protein (SERP) of Plasmodium falciparum is found within the parasitophorous vacuole. Exons 1 and 2 of the SERP gene were combined to a continous open reading frame and expressed in a cell free translation/translocation system to study translocation of the protein across membranes. The protein was found to be translocated co-translationally across canine pancreatic microsomes. This process required the presence of the signal recognition particle, and it was accompanied by cleavage of a signal peptide. We conclude that the authentic SERP is exported from the parasite cell via the endoplasmic reticulum.