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An improved embedding method for obtaining semithin and ultrathin sections from critical-point dried trachea specimens

 
: Brockmeyer, C.; Mohr, U.; Thiedemann, K.-U.

Experimental Pathology 36 (1989), Nr.4, S.233-236
ISSN: 0232-1513
Englisch
Zeitschriftenaufsatz
Fraunhofer ITA ( ITEM) ()
comparative microscopy; dimethylsulphoxide; respiratory tract; scanning electron microscopy; semithin section; transmission electron microscopy

Abstract
Critical-point dried trachea specimens were rinsed for 48h in dimethylsulphoxide before being embedded in Epon 812 to obtain semithin and ultrahin sections. Compared with methods hitherto published, this treatment leads to better resin infiltration of the specimens and better preservation of intracellular structures.

: http://publica.fraunhofer.de/dokumente/PX-17945.html