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The genotoxicity of lucidin, a natural component of Rubia Tinctorum L., and lucidineethylether, a component of ethanolic Rubia extracts

: Groth, G.; Marquardt, H.; Poginsky, B.; Westendorf, J.

Cell Biology and Toxicology 4 (1988), Nr.2, S.225-239
ISSN: 0742-2091
Fraunhofer ITA ( ITEM) ()
DNA; genotoxicity; hepatocyte; liver; lucidin; mutagenic effect; mutagenicity; rat; Rubia Tinctorum L.; short term test

The genotoxicity of lucidin was tested in a battery of short term tests. The compound was mutagenic in five Salmonella typhimurium strains without metabolic activation, but the mutagenicity was increased after addition of rat liver S9 mix. IN V79 cells, lucidin was mutagenic at the hypoxanthineguanine phosphoribosyl transferase gene locus and active at inducing DNA single-strand breaks and DNA-protein cross-links as assayed by the alkaline elution method. Lucidin also induced DNA repair synthesis in primary rat hepatocytes and transformed C3H/M2 mouse fibroblasts in culture. We also investigated lucidinethylether, which is formed from lucidin by extraction of madder roots with boiling ethanol. This compound was also mutagenic in Salmonella, but only after addition of rat liver S9 mix. Lucidinethylether was weakly mutagenic to V79 cells which were cocultivated with rat hepatocytes. The compound did not induce DNA repair synthesis in hepytocytes from untreated rats, but positive result w ere obtained when hepatocytes from rats pretreated with phenobarbital were used. We conclude that lucidin and its derivatives are genotoxic.