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1982
Journal Article
Titel
Effect of N,N-diallyl-2,2-dichloroacetamide -R-25788- on efflux and synthesis of glutathione in tobacco suspension cultures
Abstract
The low level of glutathione synthesis observed in tobacco suspension cultures grown under heterotrophic conditions was stimulated by the addition of the antidote R-25788 to the culture medium. Treatment of the cells with 10 E-5 M R-25788 enhanced the amount of gluthathione released during the whole culture period of 12 days by a factor of 1.7-1.8, without affecting the rate of efflux or the intracellular glutathione content. Stimulation of the high level of glutathione synthesis observed in photoheterophically grown suspensions was not achieved by the antidote. Whereas addition of R-25788 to the assay mixture did not increase glutathione synthesis in vitro, the specific activity of the enzymes of glutathione synthesis was enhanced to a significant extent in tobacco cells, cultured in medium containing R-25788. Therefore, R-25788 seems to stimulate gluthathione synthesis by an enhancement of the amount of enzymes involved in this process, rather than by a direct activation of pre-exist ing enzymes. (IFU)