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Title
Geltraeger zur Durchfuehrung des COMET-Assays mit Automatisierungsmoeglichkeit im Roboter-gestuetzten Analysebetrieb zur Realisierung eines Hochdurchsatz-Verfahrens
Date Issued
2007
Author(s)
Ritter, D.
Knebel, J.
Patent No
102007046615
Abstract
(A1) Die Erfindung betrifft einen Geltraeger (10) zur Durchfuehrung des COMET-Assays mit Automatisierungsmoeglichkeit im Roboter-gestuetzten Analysebetrieb zur Realisierung eines Hochdurchsatz-Verfahrens, umfassend eine Traegerplatte (12), deren Plattenoberseite (14) mindestens eine Traegerflaeche zum Aufbringen des Gels (16) aufweist. Erfindungsgemaess umfasst die Traegerflaeche einen Haftbereich (18), der derart ausgebildet ist, dass das auf der Traegerflaeche aufgebrachte Gel (16) in diesem Bereich (18) an der Plattenoberseite (14) haftet, und einen Analysenbereich (20), der derart ausgebildet ist, dass eine Analyse in diesem Bereich (20) durchfuehrbar ist.
WO 2009039822 A1 UPAB: 20090430 NOVELTY - The gel carrier (10) for performing comet assay having an automation option in a robot-supported analysis operation, comprises a carrier plate (12), whose plate top surface (14) comprises a carrier surface for the application of the gel. The carrier surface comprises a circular adhesion region (18) that is designed such that the gel applied to the carrier surface adheres to the plate top surface in the adhesion region, and an analysis region (20). The adhesion region frames the analysis region. The carrier plate consists of glass. The plate top surface is roughened in the adhesion region. DETAILED DESCRIPTION - The gel carrier (10) for performing comet assay having an automation option in a robot-supported analysis operation, comprises a carrier plate (12), whose plate top surface (14) comprises a carrier surface for the application of the gel. The carrier surface comprises a circular adhesion region (18) that is designed such that the gel applied to the carrier surface adheres to the plate top surface in the adhesion region, and an analysis region (20). The adhesion region frames the analysis region. The carrier plate consists of glass. The plate top surface is roughened in the adhesion region and has recesses in the adhesion region. The plate top surface in the adhesion region has a coating, which applies to a sufficient adhesion of the gel on the plate top surface, and is chemically pretreated with silanes. An image analysis is accessible with the analysis region by microscope or other suitable evaluation process. USE - Gel carrier for performing comet assay having an automation option in a robot-supported analysis operation for observing DNA damages in individual cells, where the comet assay is a single cell gel electrophoresis. ADVANTAGE - The gel carrier ensures reliable investigation of multiple samples, is capable of rapidly, sensitively and simply performing the comet assay with less material consumption and high throughput, can be economically and easily produced, and is re-usable.
Language
de
Patenprio
DE 102007046615 A: 20070927