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Fast detection of air contaminants using immunobiological methods

: Schmitt, K.; Bolwien, C.; Sulz, G.; Koch, W.; Dunkhorst, W.; Lödding, H.; Schwarz, K.; Holländer, A.; Klockenbring, T.; Barth, S.; Seidel, B.; Hofbauer, W.; Rennebarth, T.; Renzl, A.

Volltext urn:nbn:de:0011-n-955263 (659 KByte PDF)
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Copyright 2009 Society of Photo-Optical Instrumentation Engineers. This paper is made available as an electronic reprint (preprint) with permission of SPIE. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Erstellt am: 9.6.2009

Schmid, U. ; Society of Photo-Optical Instrumentation Engineers -SPIE-, Bellingham/Wash.:
Smart Sensors, Actuators, and MEMS IV : 4 May 2009, Dresden, Germany
Bellingham, WA: SPIE, 2009 (Proceedings of SPIE 7362)
ISBN: 0-8194-7636-6
ISBN: 978-0-8194-7636-4
ISSN: 0277-786X
Art. 736207
Conference "Smart Sensors, Actuators, and MEMS" <4, 2009, Dresden>
Konferenzbeitrag, Elektronische Publikation
Fraunhofer IPM ()
Fraunhofer IBP ()
indoor air quality; microorganism; immunobiological recognition; fluorescence; polymer substrate; electrophoretic separation

The fast and direct identification of possibly pathogenic microorganisms in air is gaining increasing interest due to their threat for public health, e.g. in clinical environments or in clean rooms of food or pharmaceutical industries. We present a new detection method allowing the direct recognition of relevant germs or bacteria via fluorescence-labeled antibodies within less than one hour. In detail, an air-sampling unit passes particles in the relevant size range to a substrate which contains antibodies with fluorescence labels for the detection of a specific microorganism. After the removal of the excess antibodies the optical detection unit comprising reflected-light and epifluorescence microscopy can identify the microorganisms by fast image processing on a single-particle level. Fir st measurements with the system to identify various test particles as well as interfering influences have been performed, in particular with respect to autofluorescence of dust particles. Specific antibodies for the detection of Aspergillus fumigatus spores have been established. The biological test system consists of protein A-coated polymer particles which are detected by a fluorescence-labeled IgG. Furthermore the influence of interfering particles such as dust or debris is discussed.