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3D-Röntgen-Mikrocomputertomographie und optische Kohärenztomographie als Methoden zur Lagebestimmung des Blastoderms im unbebrüteten Hühnerei

3D-X-ray micro computed tomography and optical coherence tomography as methods for the localization of the germinal disk in the newly laid unincubated chicken egg
: Bartels, T.; Fischer, B.; Krueger, P.; Koch, E.; Ryll, M.; Krautwald-Junghanns, M.E.


Deutsche Tierärztliche Wochenschrift 115 (2008), Nr.5, S.182-188
ISSN: 0341-6593
Fraunhofer IZFP, Institutsteil Dresden ( IKTS-MD) ()

The routine culling of the male offspring of hybrid layer type chickens is met Summary with increasing public disapproval for both ethical and legal reasons. Until now practice-oriented methods for reliable sex diagnosis prior to hatch could not be developed. Molecular genetical analysis of blastodermic cells can be used for sex determination in unincubated eggs; however, knowledge of the precise localization of the germinal disc is crucial for the extraction of a carefully directed cell biopsy. In principle, 3D-X-ray micro computed tomography (3D-CT) has been proven a suitable method to localize the germinal disk in the unincubated egg without damaging the egg shell. No negative effects on embryogenesis and hatching rate of irradiated hatching eggs were established. The pictorial representation of the germinal disk using optical coherence tomography (OCT) failed in the unopened egg. The egg shell formed an impenetrable barrier for the currently available measuring method which utilized near infrared (NIR) wavelength regions. After opening the egg shell, the germinal disk could be visualized without any difficulties. In conclusion, technical possibilities for localization of the germinal disk in the unincubated egg already exist, but regarding technical parameters, the procedures have to be adapted to the specific purpose.