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Noninvasive time-dependent cytometry monitoring by digital holography

: Kemmler, M.; Fratz, M.; Giel, D.; Saum, N.; Brandenburg, A.; Hoffmann, C.

Postprint urn:nbn:de:0011-n-657501 (799 KByte PDF)
MD5 Fingerprint: dbefc46fd8d7a34b85386c8e0d2c42d2
Copyright 2007 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Erstellt am: 13.8.2010

Journal of biomedical optics 12 (2007), Nr.6, Art. 064002, 10 S.
ISSN: 1083-3668
ISSN: 1560-2281
Zeitschriftenaufsatz, Elektronische Publikation
Fraunhofer IPM ()
digital holographic microscopy; phase measurement; reference chart; cell swelling and shrinking; apoptosis; cell analysis

Using a digital holographic microscope setup, it is possible to measure dynamic volume changes in living cells. The cells were investigated time-dependently in transmission mode for different kinds of stimuli affecting their morphology. The measured phase shift was correlated to the cellular optical thickness, and then of the cell volume as well as the refractive index were calculated and interpreted. For the characterization of the digital holographic microscope setup, we have developed a transparent three-dimensional (3-D) reference chart that can be used as a lateral resolution chart and step-height resolution chart included in one substrate. For the monitoring of living cells, a biocompatible and autoclavable flow chamber was designed, which allows us to add, exchange, or dilute the fluid within the flow chamber. An integrated changeable coverslip enables inverse microscopic applications. Trypsinization, cell swelling and shrinking induced by osmolarity changes, and apoptosis served as model processes to elucidate the potential of the digital holographic microscopy (DHM).