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Prolonged storage of purified granulocyte concentrates: Introduction of a new purification method

: Klinkmann, Gerd; Doss, Fanny; Goudeva, Lilia; Doss, Sandra; Blasczyk, Rainer; Milej, Magdalena; Koch, Stephanie; Mitzner, Steffen; Altrichter, Jens

Volltext ()

Transfusion 62 (2022), Nr.1, S.194-204
ISSN: 0041-1132
ISSN: 1537-2995
Zeitschriftenaufsatz, Elektronische Publikation
Fraunhofer IZI ()
Granulocyte; Reinigung; Transfusion; Transfusion medicine

Background: Use of donor granulocyte concentrate (GC) has been limited due to its short storage time of 6-24 h, which is partially due to residual red blood cells (RBCs) and platelets and the resulting lactate production leading to an acidotic milieu. To increase this storage time, we developed a closed system procedure compatible with standard blood bank technologies to remove RBC and platelets and to enrich the GC. Methods: Standard GCs (sGCs) were sedimented, washed twice with 0.9% sodium chloride (NaCl), and resuspended in blood group-identical fresh frozen plasma. The resulting purified GCs (pGCs) were then stored in platelet bags at a cell concentration of about 5 × 107 ± 1.8 × 107 leukocytes/ml without agitation at room temperature for up to 72 h. Cell count and viability, pH, blood gases, phagocytosis, and oxidative burst were monitored daily. Results: A significant reduction in RBC (98%) through sedimentation, and platelets (96%) by washing, purified the white blood cell (WBC) population and enriched the granulocytes to 96% of the WBC in the pGC. After 72 h of storage, over 90% of the initial WBC count of pGC remained, was viable (≥97%), and the granulocytes exhibited a high phagocytosis and oxidative burst functionality, comparable to sGC after 24 h. Conclusion: Purification extends the maximum storage period of GC from 24 to 72 h and may therefore improve the availability of GC and its clinical use.