Options
2020
Journal Article
Titel
Impact of self-assembling peptides in remineralization of early enamel lesions adjacent to orthodontic brackets detected by bioluminescence measurements
Alternative
Einfluss von Self-assembling Peptiden auf die Remineralisation initialkari ser L sionen am Bracketrand nachgewiesen mittels Biolumineszenzverfahren
Abstract
Purpose Enamel demineralization can occur as a side effect during orthodontic treatment with fixed appliances. This study aimed to evaluate the efficacy of the self-assembling peptide P11-4 for remineralization combined with fluorides, compared to application of fluoride varnish alone. De- and remineralization was assessed using a novel bioluminescence technology measuring free calcium ions in initial lesions. Methods Orthodontic brackets were bonded on human enamel samples and white spot lesions were created. 117 samples were allocated randomly into 3 groups: Group I = no treatment, group II = single application of fluoride varnish (22,600 ppm), group III = treatment with P11-4 following a single application of fluoride varnish. Bioluminescence and Quantitative light-induced fluorescence (QLF) measurements were performed at baseline, after demineralization and after storage in artificial saliva for 7, 30 and 90 days. Results After demineralization, all areas showed loss of fluorescence (median F -9.44 %). The median F differed significantly between all groups at all investigation times (p < 0.000001). The proportion of samples without luminescence spots were 25.0 % in group I, 21.4 % in group II and 30.8 % in group III after 90 days. Conclusions Application of P11-4 combined with fluoride varnish was superior to the use of fluorides alone for remineralization of enamel adjacent to brackets. The bioluminescence system was shown to be a promising tool for digital demonstration of de- and remineralization.