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2005
Conference Paper
Titel
Perfusion chamber for cell tests with micropatterned surface
Alternative
Perfusionskammer für Zelltests mit mikrotexturierten Oberflächen
Abstract
Character and direction of cell growth can be influenced by localized surface modification. This is of great importance in both scientific and practical respects (cell micro arrays, tissue engineering). Laser-structuring of polymeric surfaces in microscale and nanoscale is of particular interest. Latest publications show that combining physical, chemical and topographical cues result in one combined biological effect. A modular bioreactor for perfusion cultures has been designed to support the analysis of these technologies. Its unique feature is a selective processing of the polystyrene cell contact surfaces before mounting the perfusion chamber. This preprocessing includes an isolated structuring and modification of the polymeric surface to adjust properties such as the concentration of nitrogen, surface tension, wetting etc. The design of the perfusion chamber encourages a reproducible culturing. It meets the special requirements of laboratory use and it makes possible the above mentioned preparations and analysis. In particular, the perfusion was optimized by extensive modelling in ANSYS-FLOTRAN.