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Powering artificial enzymatic cascades with electrical energy

: Al-Shameri, Ammar; Petrich, Marie-Christine; junge Puring, Kai; Apfel, Ulf-Peter; Nestl, Bettina M.; Lauterbach, Lars

Volltext ()

Angewandte Chemie. International edition 59 (2020), Nr.27, S.10929-10933
ISSN: 1433-7851
ISSN: 0044-8249
ISSN: 0570-0833
ISSN: 1521-3773
Zeitschriftenaufsatz, Elektronische Publikation
Fraunhofer UMSICHT Oberhausen ()
electrochemical biocatalysis; hydrogenases; imine reductases; isotopic labeling; N-heterocycles

We have developed a scalable platform that employs electrolysis for an in vitro synthetic enzymatic cascade in a continuous flow reactor. Both H2 and O2 were produced by electrolysis and transferred through a gas‐permeable membrane into the flow system. The membrane enabled the separation of the electrolyte from the biocatalysts in the flow system, where H2 and O2 served as electron mediators for the biocatalysts. We demonstrate the production of methylated N‐heterocycles from diamines with up to 99 % product formation as well as excellent regioselective labeling with stable isotopes. Our platform can be applied for a broad panel of oxidoreductases to exploit electrical energy for the synthesis of fine chemicals.