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In vitro investigation of the geometric contraction behavior of chemo-mechanical P-protein aggregates (forisomes)

 
: Schwan, S.; Fritzsche, M.; Cismak, A.; Heilmann, A.; Spohn, U.

:

Biophysical chemistry 125 (2007), Nr.2-3, S.444-452
ISSN: 0301-4622
Englisch
Zeitschriftenaufsatz
Fraunhofer IWM ()
chemo-mechanical protein aggregate; forisome; contracting behavior; Ca2+ induced contraction; pH induced contraction; geometric parameter

Abstract
We investigated the contracting behavior of forisomes from Vicia faba by carrying out precise measurements of their changing geometric parameters in vitro in the absence and in the presence of dissolved oxygen. Furthermore, we investigated the fine structure of forisomes by scanning electron microscopy. For the first time, single forisomes were titrated with Ca2+, protons, and hydroxide ions recording the complete progression of their contractions. An apparent Ca2+-binding constant of (22 ± 3) µM was calculated from two complete titration curves. The forisomes also contracted in the presence of Ba2+ and Sr2+ ions, but the amplitudes of contraction were smaller under the same measuring conditions. The time taken to change from the longitudinally expanded into the longitudinally contracted state was up to 2 s shorter in 10 mM Ca2+ in comparison to 0.2 mM Ca2+. However, the contraction time was prolonged by decreasing the Ca2+ concentration. In the absence of dissolved oxygen, the transition between the two final states of the forisomes was almost reversible and the amplitude of contraction remained almost constant during the first 25 contraction cycles. In the presence of dissolved oxygen the forisomes denaturated after a few cycles and lost their ability to contract, just after only a few cycles with 10 min in the contracted state. Denaturation of the forisomes occurred appreciably in the contracted state. We propose a cycle process to explain the thermodynamic basis of the Ca2+-induced contraction and its reversal by EDTA. Reducing the pH-value from 7.3 to 4.0 caused the forisomes to shorten by approximately 15%, while increasing the pH to 11.0 caused them to shorten by 28 to 30%. In both cases, the increases of the forisomes volume were greater than during the Ca2+ induced contraction. The pH values of 4.7 ± 0.3, and 10.2 ± 0.2 marked the inflection points of the acid base titration of different forisomes.

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