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Characterization of photosynthetically active duckweed (Wolffia australiana) in vitro culture by Respiration Activity Monitoring System (RAMOS)

: Rechmann, H.; Friedrich, A.; Forouzan, D.; Barth, S.; Schnabl, H.; Biselli, M.; Boehm, R.


Biotechnology letters 29 (2007), Nr.6, S.971-977
ISSN: 0141-5492 (Print)
ISSN: 1573-6776 (Online)
Fraunhofer IME ()

The feasibility of oxygen transfer rate (OTR) measurement to non-destructively monitor plant propagation and vitality of photosynthetically active plant in vitro culture of duckweed (Wolffia australiana, Lemnaceae) was tested using Respiration Activity Monitoring System (RAMOS). As a result, OTR proofed to be a sensitive indicator for plant vitality. The culture characterization under day/night light conditions, however, revealed a complex interaction between oxygen production and consumption, rendering OTR measurement an unsuitable tool to track plant propagation. However, RAMOS was found to be a useful tool in preliminary studies for process development of photosynthetically active plant in vitro cultures.