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Diffraction-based technology for the monitoring of contraction dynamics in 3D and 2D tissue models

: Harzic, Ronan le; Meiser, Ina; Neubauer, Julia C.; Riemann, Iris; Schiffer, Michael; Stracke, Frank; Zimmermann, Heiko

Volltext urn:nbn:de:0011-n-5824060 (3.4 MByte PDF)
MD5 Fingerprint: 89dd993cdf258ad827a51cb661e772e4
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Erstellt am: 20.10.2020

Biomedical optics express. Online journal 11 (2020), Nr.2, S.517-532
ISSN: 2156-7085
Zeitschriftenaufsatz, Elektronische Publikation
Fraunhofer IBMT ()

We present a novel optical device developed for the monitoring of dynamic behavior in extended 3D-tissue models in various culture environments based on variations in their speckle patterns. The results presented point out the benefit of the technology in terms of detection, accuracy, sensitivity and a reasonable read-out speed as well as reproducibility for the measurements and monitoring of cardiac contractions. We show that the optical read-out technology is suitable for long time monitoring and for drug screening. The method is discussed and compared to other techniques, in particular calcium imaging. The device is flexible and easily adaptable to 2D and 3D-tissue model screenings using different culture environments. The technology can be parallelized for automated read-out of different multi-well-plate formats.