Fraunhofer-Gesellschaft

Publica

Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

To beat the heat - engineering of the most thermostable pyruvate decarboxylase to date

 
: Sutiono, S.; Satzinger, K.; Pick, A.; Carsten, J.; Sieber, V.

:

RSC Advances 9 (2019), Nr.51, S.29743-29746
ISSN: 2046-2069
Englisch
Zeitschriftenaufsatz
Fraunhofer IGB ()

Abstract
Pyruvate decarboxylase (PDC) is a key enzyme for the production of ethanol at high temperatures and for cell-free butanol synthesis. Thermostable, organic solvent stable PDC was evolved from bacterial PDCs. The new variant shows >1500-fold-improved half-life at 75 °C and >5000-fold-increased half-life in the presence of 9 vol% butanol at 50 °C.

: http://publica.fraunhofer.de/dokumente/N-564891.html