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Hitze stabilisiert Malariaimpfstoffkandidaten in komplexen Proteinextrakten

Heat stabilizes malaria vaccine candidates in complex protein extracts
: Buyel, J.F.


Biospektrum 25 (2019), Nr.2, S.163-166
ISSN: 0947-0867
ISSN: 1868-6249
Fraunhofer IME ()

Plants can be an alternative to mammalian cell cultures in terms of vaccine production due to low upstream production costs and a good safety profile. However, product purification can be challenging because of abundant host cell proteins (HCPs) in raw plant extracts. Here we report the optimization of blanching, a heat-based method, that allowed to remove over 90 percent of HCPs and to stabilize a malaria vaccine candidate by carefully adjusting the temperature in the step and combined it with solid-liquid separation and chromatographic purification.