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2019
Journal Article
Titel
Hitze stabilisiert Malariaimpfstoffkandidaten in komplexen Proteinextrakten
Alternative
Heat stabilizes malaria vaccine candidates in complex protein extracts
Abstract
Plants can be an alternative to mammalian cell cultures in terms of vaccine production due to low upstream production costs and a good safety profile. However, product purification can be challenging because of abundant host cell proteins (HCPs) in raw plant extracts. Here we report the optimization of blanching, a heat-based method, that allowed to remove over 90 percent of HCPs and to stabilize a malaria vaccine candidate by carefully adjusting the temperature in the step and combined it with solid-liquid separation and chromatographic purification.