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Effects of commensal biofilms on a peri-implant mucosa in a three dimensional model

: Mikolai, C.; Ingendoh-Tsakmakidis, A.; Winkel, A.; Kommerein, N.; Falk, C.S.; Rossi, Angela; Walles, Heike; Stiesch, M.


Biomedizinische Technik 64 (2019), Nr.S1, S.63
ISSN: 0013-5585
ISSN: 1862-278X
German Society for Biomaterials (Annual Meeting) <2018, Braunschweig>
Fraunhofer ISC ()
Biofilm; Implantat

A symbiotic microbial community of commensals exists during oral health. Pathogenic bacteria are able to induce dysregulation of hostmicrobiota homeostasis and a dysbiotic biofilm formation can cause peri-implantitis, a chronic infection. Little is known about the role of commensal and pathogenic bacteria in the regulation of the balance between host tolerance and inflammation. Previous studies have indicated that commensal bacteria are related to disease prevention, but details about the modulatory effects are still missing. Moreover, knowledge about the influence of implant material on host-microbe interaction is limited. Thus, our aim was to investigate the effects of a commensal monospecies Streptoccocus oralis biofilm and a commensal four-species biofilm (S. oralis, Actinomyces naeslundii, Veillonella dispar, Porphyromonas gingivalis) in our novel implant-mucosa model. The three dimensional in vitro model combines the three components oral mucosa, implant material and oral biofilm. After S. oralis challenge, a morphological difference at the implant area was observed in comparison to the farther or unchallenged mucosa. In addition, culture with S. oralis biofilm led to an altered cytokine/chemokine secretion. This indicates a hyporesponsiveness of the tissue which can protect it from inflammatory destruction. However, in the in vivo situation a multispecies biofilm is present. Therefore, we integrated a commensal four-species biofilm in our model to reduce the gap to the in vivo situation. The four-species biofilm challenged mucosa showed no morphological difference to the unchallenged mucosa. This illustrates that the effects of commensal mono- or multispecies biofilms are different and the interaction of bacteria species can be important regarding tissue response. The novel 3D implant-mucosa model opens the opportunity for various in vitro studies of host-microbe interactions for implant improvement.