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Roquin targets mRNAs in a 3′-UTR-specific manner by different modes of regulation

 
: Essig, Katharina; Kronbeck, Nina; Guimaraes, Joao C.; Lohs, Claudia; Schlundt, Andreas; Hoffmann, Anne; Behrens, Gesine; Brenner, Sven; Kowalska, Joanna; Lopez-Rodriguez, Cristina; Jemielity, Jacek; Reiche, Kristin; Holtmann, Helmut; Hackermüller, Jörg; Sattler, Michael; Zavolan, Mihaela; Heissmeyer, Vigo

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Volltext ()

Nature Communications 9 (2018), Art. 3810, 17 S.
ISSN: 2041-1723
Englisch
Zeitschriftenaufsatz, Elektronische Publikation
Fraunhofer IZI ()

Abstract
The RNA-binding proteins Roquin-1 and Roquin-2 redundantly control gene expression and cell-fate decisions. Here, we show that Roquin not only interacts with stem–loop structures, but also with a linear sequence element present in about half of its targets. Comprehensive analysis of a minimal response element of the Nfkbid 3′-UTR shows that six stem–loop structures cooperate to exert robust and profound post-transcriptional regulation. Only binding of multiple Roquin proteins to several stem–loops exerts full repression, which redundantly involved deadenylation and decapping, but also translational inhibition. Globally, most Roquin targets are regulated by mRNA decay, whereas a small subset, including the Nfat5 mRNA, with more binding sites in their 3′-UTRs, are also subject to translational inhibition. These findings provide insights into how the robustness and magnitude of Roquin-mediated regulation is encoded in complex cis-elements.

: http://publica.fraunhofer.de/dokumente/N-534867.html