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Lipid phenotyping of lung epithelial lining fluid in healthy human volunteers

 
: Brandsma, Joost; Goss, Victoria M.; Yang, Xian; Bakke, Per S.; Caruso, Massimo; Chanez, Pascal; Dahlén, Sven-Erik; Fowler, Stephen J.; Horváth, Ildikó; Krug, Norbert; Montuschi, Paolo; Sanak, Marek; Sandström, Thomas; Shaw, Dominick E.; Chung, Kian Fan; Singer, Florian; Fleming, Louise J.; Sousa, Ana R.; Pandis, Ioannis; Bansal, Aruna T.; Sterk, Peter J.; Djukanović, Ratko; Postle, Anthony D.

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Volltext urn:nbn:de:0011-n-5240417 (1.7 MByte PDF)
MD5 Fingerprint: 92b8f2f3026f69009bf7f387ea61b788
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Erstellt am: 15.12.2018


Metabolomics 14 (2018), Nr.10, Art. 123, 12 S.
ISSN: 1573-3882
ISSN: 1573-3890
Englisch
Zeitschriftenaufsatz, Elektronische Publikation
Fraunhofer ITEM ()
induced sputum; epithelial lining fluid; pulmonary surfactant; lipid metabolism; lipidomics; mass spectrometry; weight status; Asthma; chronic obstructive pulmonary disease; pulmonary fibrosis

Abstract
Background
Lung epithelial lining fluid (ELF)—sampled through sputum induction—is a medium rich in cells, proteins and lipids. However, despite its key role in maintaining lung function, homeostasis and defences, the composition and biology of ELF, especially in respect of lipids, remain incompletely understood.
Objectives
To characterise the induced sputum lipidome of healthy adult individuals, and to examine associations between different ELF lipid phenotypes and the demographic characteristics within the study cohort.
Methods
Induced sputum samples were obtained from 41 healthy non-smoking adults, and their lipid compositions analysed using a combination of untargeted shotgun and liquid chromatography mass spectrometry methods. Topological data analysis (TDA) was used to group subjects with comparable sputum lipidomes in order to identify distinct ELF phenotypes.
Results
The induced sputum lipidome was diverse, comprising a range of different molecular classes, including at least 75 glycerophospholipids, 13 sphingolipids, 5 sterol lipids and 12 neutral glycerolipids. TDA identified two distinct phenotypes differentiated by a higher total lipid content and specific enrichments of diacyl-glycerophosphocholines, -inositols and -glycerols in one group, with enrichments of sterols, glycolipids and sphingolipids in the other. Subjects presenting the lipid-rich ELF phenotype also had significantly higher BMI, but did not differ in respect of other demographic characteristics such as age or gender.
Conclusions
We provide the first evidence that the ELF lipidome varies significantly between healthy individuals and propose that such differences are related to weight status, highlighting the potential impact of (over)nutrition on lung lipid metabolism.

: http://publica.fraunhofer.de/dokumente/N-524041.html