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Anti-inflammatory effects of Phaeodactylum tricornutum extracts on human blood mononuclear cells and murine macrophages

 
: Neumann, Ulrike; Louis, Sandrine; Gille, Andrea; Derwenskus, Felix; Schmid-Staiger, Ulrike; Briviba, Karlis; Bischoff, Stephan C.

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Journal of applied phycology 30 (2018), Nr.5, S.2837-2846
ISSN: 0921-8971
International Society for Applied Phycology (Congress) <6, 2017, Nantes>
Englisch
Zeitschriftenaufsatz, Konferenzbeitrag
Fraunhofer IGB ()
Cell viability; Cytokines; Diatom; MAP kinases; mRNA expression; NF-κB

Abstract
The microalgae Phaeodactylum tricornutum (PT) is known for its high content of omega-3 fatty acids, which are known to attenuate inflammation. Additionally, this microalga contains other nutrients such as carbohydrates, vitamins, proteins, and carotenoids and therefore could be of interest for animal and human nutrition. Here, we investigated the effects of hexane, ethanolic, and aqueous extracts on lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMCs) and on the murine macrophage cell line RAW 264.7. Further, the cytotoxicity of the extracts was studied using the MTT assay. The production of pro-inflammatory cytokines was significantly inhibited by the ethanolic and aqueous P. tricornutum extracts but not by the hexane extract. Both at the mRNA and at the protein levels, the aqueous extract inhibited LPS-induced IL-6, IL-1β, TNFα, and COX-2 expression and release by up to 96% (mRNA) and 79% (protein) in a dose-dependent manner. Compared to the aqueous extract, the ethanolic extract was less effective in cytokine inhibition. The production of nitric oxide in RAW 264.7 cells was significantly reduced by all extracts. We showed that the anti-inflammatory effect of P. tricornutum is exerted through inhibition of nuclear factor-κB activation and dependent on the mitogen-activated protein (MAP) kinase pathway. Our data indicate anti-inflammatory effects of the aqueous P. tricornutum extract and provide a basis information on the safety and potential health benefits of P. tricornutum usage for future animal and human nutrition.

: http://publica.fraunhofer.de/dokumente/N-519800.html