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Photoinduced cleavage and hydrolysis of o-nitrobenzyl linker and covalent linker immobilization in gelatin methacryloyl hydrogels

 
: Claaßen, Christiane; Claaßen, Marc H.; Gohl, Fabian; Tovar, Günter; Borchers, Kirsten; Southan, Alexander

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Macromolecular bioscience 18 (2018), Nr.9, Art. 1800104, 13 S.
ISSN: 1616-5187
ISSN: 1616-5195
Englisch
Zeitschriftenaufsatz
Fraunhofer IGB ()
gelatin methacryloy; lLC-MS; photocleavable linker; stimuli-responsive release; streptavidin-coupled HRP

Abstract
Light‐induced release systems can be triggered remotely and are of interest for many controlled release applications due to the possibility for spatio‐temporal release control. In this study a biotin‐functionalized photocleavable macromer is incorporated with an o‐nitrobenzyl moiety into gelatin methacryloyl(‐acetyl) hydrogels via radical cross‐linking. Stronger immobilization of streptavidin‐coupled horseradish peroxidase occurs in linker‐functionalized hydrogels compared to pure gelatin methacryloyl(‐acetyl) hydrogels, and a controlled release of the streptavidin conjugate upon UV‐irradiation is possible. Liquid chromatography coupled to mass spectrometry (LC‐MS) analysis of aqueous linker solutions allows the identification of the main cleavage products and the cleavage kinetics. Thus, it is shown that a significant hydrolysis of the linker occurs at 37 °C. Nevertheless the system reported here is a promising controlled release scaffold for proteins and application in tissue engineering, if background releases of the immobilized drug are tolerable.

: http://publica.fraunhofer.de/dokumente/N-512433.html