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Surface topography guides morphology and spatial patterning of induced pluripotent stem cell colonies

 
: Abagnale, G.; Sechi, A.; Steger, M.; Zhou, Q.; Kuo, C.-C.; Aydin, G.; Schalla, C.; Müller-Newen, G.; Zenke, M.; Costa, I.G.; Rijn, P. van; Gillner, A.; Wagner, W.

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Volltext ()

Stem cell reports 9 (2017), Nr.2, S.654-666
ISSN: 2213-6711
Englisch
Zeitschriftenaufsatz, Elektronische Publikation
Fraunhofer ILT ()

Abstract
The relevance of topographic cues for commitment of induced pluripotent stem cells (iPSCs) is largely unknown. In this study, we demonstrate that groove-ridge structures with a periodicity in the submicrometer range induce elongation of iPSC colonies, guide the orientation of apical actin fibers, and direct the polarity of cell division. Elongation of iPSC colonies impacts also on their intrinsic molecular patterning, which seems to be orchestrated from the rim of the colonies. BMP4-induced differentiation is enhanced in elongated colonies, and the submicron grooves impact on the spatial modulation of YAP activity upon induction with this morphogen. Interestingly, TAZ, a YAP paralog, shows distinct cytoskeletal localization in iPSCs. These findings demonstrate that topography can guide orientation and organization of iPSC colonies, which may affect the interaction between mechanosensors and mechanotransducers in iPSCs.

: http://publica.fraunhofer.de/dokumente/N-507339.html