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An in vitro coculture system composed of THP-1 cells and 3D reconstructed human epidermis to assess activation of dendritic cells by sensitizing chemicals

: Schellenberger, M.T.; Bock, U.; Hennen, J.; Gröber-Becker, F.; Walles, Heike; Blömeke, B.

Naunyn-Schmiedebergs archives of pharmacology 391 (2018), Supplement 1, S.S49
ISSN: 0028-1298
ISSN: 1432-1912
German Society for Experimental and Clinical Pharmacology and Toxicology (DGPT Annual Meeting) <84, 2018, Göttingen>
Association of the Clinical Pharmacology Germany (VKliPha Annual Meeting) <20, 2018, Göttingen>
Fraunhofer ISC ()
Zellen; Haut

Dendritic cell activation is a key event of the adverse outcome pathway (AOP) for skin sensitization. In an attempt to integrate the impact of the skin environment created by stratified keratinocyte layers on the activation of dendritic cells, we investigated whether available 3D reconstructed human epidermis (RHE) models can be cocultured together with THP-1 cells, as surrogate dendritic cells, to assess chemical-induced dendritic cell activation in vitro. For this purpose, THP-1 cells were placed underneath the RHE, which was topically exposed to increasing concentrations of the model sensitizer eugenol (dissolved in aceton-olive oil, 4:1). Cell surface expression of the costimulatory molecule CD86 and the adhesion molecule CD54 on THP-1 cells were analyzed by flow cytometry. Preliminary results are promising and we conclude that this approach may allow the investigation of compounds with varying physicochemical properties and exposure at the air liquid interface.